Anti-OsIRE1 Rabbit Polyclonal Antibody for WB, ELISA - Q7XIT1

Core Product Specifications and Parameters

Biological Background: OsIRE1 Function and Localization

• OsIRE1 is a serine/threonine-protein kinase/endoribonuclease that plays a pivotal role in the endoplasmic reticulum (ER) stress response in rice.
• It senses unfolded proteins in the ER lumen through its N-terminal domain, triggering auto-activation and downstream signaling.
• The activated endoribonuclease domain splices bZIP50 mRNA, generating a potent unfolded-protein response (UPR) transcriptional activator.
• This splicing event leads to the induction of UPR target genes, including luminal-binding protein (BiP) chaperones, to restore ER homeostasis.
• OsIRE1 is localized to the endoplasmic reticulum membrane, consistent with its role in ER stress sensing.
• Expression is observed in various rice tissues, including roots, nodes, internodes, leaf sheaths, leaf blades, and young and mature ears.
• The protein contains multiple functional domains: a signal sequence, transmembrane regions, kinase, and endoribonuclease domains, and is classified as a multifunctional enzyme with ATP-binding and nucleotide-binding activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 436-650, covering part of the kinase and endoribonuclease domains; consider validating recognition of both domains in your specific assay.
• For Western blot (WB), a band near 99kDa is expected; optimize sample preparation with appropriate reducing agents and detergent lysis.
• In ELISA applications, use the recombinant immunogen or endogenous protein from rice tissues known to express OsIRE1 as positive controls.
• As a polyclonal antibody, it may exhibit cross-reactivity with related proteins; include knockout or knockdown controls to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For ER stress and UPR pathway research, CamelBio offers a range of validated antibodies and ancillary reagents, including this anti-OsIRE1 rabbit polyclonal antibody. Our supply capabilities extend to bulk orders, rare target antibodies, and customized sourcing to accelerate your rice stress biology and diagnostic development projects.