Anti-OsGRF4 Polyclonal Antibody for WB and ELISA - Q6ZIK5

Core Product Specifications and Parameters

Biological Background: OsGRF4 Function and Localization

• Transcription activator regulating meristematic function in leaves, stems, and inflorescences. Related references: PMID:24532604
• Positive regulator of grain size; promotes cell division and expansion to increase grain length and width. Related references: PMID:26187814, PMID:26936408, PMID:27107174
• Activates expression of cell proliferation genes and expansin genes for cell expansion. Related references: PMID:26187814, PMID:26936408, PMID:27250747
• May enhance grain size via brassinosteroid response activation; part of miRNA396-GRF-GIF regulatory module. Related references: PMID:27107174, PMID:27250747, PMID:27250749
• Localized to the nucleus, consistent with its role as a transcription factor.
• Expressed in stems and panicles, aligning with roles in vegetative and reproductive growth. Related references: PMID:26187814, PMID:27107174, PMID:27250747, PMID:27250749

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 42 kDa; consider using protein extracts from rice stem or panicle tissues, where OsGRF4 is expressed.
• The recombinant immunogen (aa 120-394) can serve as a positive control or standard in ELISA experiments.
• Cross-reactivity is reported with Oryza sativa; test other species as necessary.
• Optimize antibody dilution and blocking conditions for your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, offering a broad range of antibodies and ancillary reagents for plant biology and agricultural research. This anti-OsGRF4 polyclonal antibody supports studies on grain development, crop yield improvement, and rice functional genomics. Contact us for bulk supply, validated antibody pairs, or custom raw material sourcing solutions tailored to your project needs.