Anti-OSGEPL1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9H4B0

Core Product Specifications and Parameters

Biological Background: OSGEPL1 Function and Localization

• OSGEPL1, also referred to as Qri7, OSGEPL, or tRNA N6-adenosine threonylcarbamoyltransferase, is a mitochondrial enzyme essential for tRNA modification.
• The protein catalyzes the threonylcarbamoylation of adenosine at position 37 (t6A37) in mitochondrial tRNAs that decode ANN codons, ensuring proper codon-anticodon pairing and translation fidelity.
• As a member of the t(6)A synthase family, OSGEPL1 likely functions as a transferase, transferring the threonylcarbamoyl moiety from threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.
• It is localized to the mitochondrion and is involved in mitochondrial genome maintenance, contributing to overall mitochondrial homeostasis.
• Post-translational modifications include acetylation, and alternative splicing may produce multiple isoforms.
• The protein is widely expressed across tissues, with highest levels in the pituitary gland, prostate, rectum, and uterus.
• UniProt keywords highlight its roles in tRNA processing, acyltransferase and transferase activities, metal-binding, and the presence of a transit peptide.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–270 of human OSGEPL1, likely targeting the N-terminal region; epitope accessibility may vary across applications.
• In Western blotting (WB), a band near 45 kDa is expected; use mitochondrial extracts or positive control lysates from human, mouse, or rat cells for validation.
• For IHC-P, consider optimizing fixation and antigen retrieval conditions, and include tissue sections from high-expression sites such as pituitary gland, prostate, rectum, or uterus.
• ELISA assays can employ recombinant OSGEPL1 protein or peptide fragments; titration of the antibody is recommended for optimal signal-to-noise ratio.
• Since the antibody cross-reacts with mouse and rat, it is suitable for comparative studies; always confirm specificity in the species and sample type of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including this polyclonal antibody targeting the mitochondrial tRNA modification enzyme OSGEPL1. Our portfolio covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, supporting your work in mitochondrial biology and translational research from concept to clinic.