Anti-OsGBSSⅠ Rabbit Polyclonal Antibody for WB and ELISA - Q0DEV5

Core Product Specifications and Parameters

Biological Background: OsGBSSⅠ (Granule-bound starch synthase I) Function and Localization

• The target protein is Granule-bound starch synthase 1, chloroplastic/amyloplastic (GBSS-I), also known as OsGBSSⅠ, Waxy, or Wx, and is encoded by the WAXY gene in Oryza sativa subsp. japonica.
• GBSS-I is essential for amylose synthesis; it is specifically required for the production of amylose in the rice endosperm.
• As a member of the glycosyltransferase family, it catalyzes the transfer of glucose moieties to elongating amylose chains within starch granules.
• Subcellularly, GBSS-I is localized to plastids, predominantly in chloroplasts and amyloplasts, where starch biosynthesis occurs.
• The protein contains a transit peptide directing it to plastids, and its mature form exhibits an intramolecular disulfide bond; it has been classified as an allergen.
• The calculated molecular weight of the precursor is approximately 66 kDa (66,476 Da), consistent with the observed protein weight of 66 kDa.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against an N-terminal fragment (amino acids 1-100) of OsGBSSⅠ. Researchers may consider validating its reactivity in their specific Oryza sativa cultivars or tissue extracts, particularly endosperm where GBSS-I expression is highest.
• For Western blotting, a band around 66 kDa is expected; however, sample preparation (e.g., reducing conditions) may affect detection due to potential disulfide bond formation.
• For ELISA applications, optimization of coating antigen concentration and detection conditions using known positive and negative controls is advisable.
• Cross-reactivity with other rice starch synthase isoforms or related proteins cannot be excluded; consider sequence alignment of the immunogen region to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials for plant biology and starch metabolism research, including validated antibody pairs, optimized polyclonal antibodies like this anti-OsGBSSⅠ, and bulk ancillary reagents. This antibody supports research into amylose synthesis, rice grain quality, and related agricultural diagnostics. For rare target raw-material sourcing or custom antibody development, CamelBio serves as a reliable partner from concept to clinic.