Anti-OsFD2 Polyclonal Antibody for WB and ELISA - Q5Z4G3

Core Product Specifications and Parameters

Biological Background: OsFD2 Function and Localization

• OsFD2 (OsbZIP55) is a basic leucine zipper (bZIP) transcription factor encoded by the rice (Oryza sativa) genome.
• According to UniProt annotation, OsFD2 is implicated in the abscisic acid signaling pathway, suggesting involvement in plant stress responses.
• The protein contains a coiled-coil domain and a DNA-binding region, characteristic of bZIP transcription factors, enabling dimerization and sequence-specific DNA recognition.
• Subcellular localization is predicted to be nuclear, consistent with its role in transcriptional regulation.
• The calculated molecular weight of the unprocessed protein is approximately 18.3 kDa (18259 Da), aligning with the observed product weight.
• Keywords from UniProt include 'Abscisic acid signaling pathway', 'Coiled coil', 'DNA-binding', 'Nucleus', 'Reference proteome', 'Transcription', and 'Transcription regulation'.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 100 amino acids of OsFD2, which may confer specificity for this region; full-length reactivity should be confirmed in the relevant experimental system.
• For Western blot applications, a band around 18 kDa is anticipated; consider using whole-cell lysates from rice tissues and validate with recombinant protein or genetic controls.
• In ELISA setups, the recombinant protein or the immunogen peptide can serve as a coating antigen; optimization of coating concentration and antibody dilution is recommended.
• As with any polyclonal antibody, lot-to-lot consistency may vary; consider testing each lot in a pilot experiment before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive resource for IVD raw materials, empowering diagnostic manufacturers and research laboratories with a streamlined supply chain from concept to market. This anti-OsFD2 polyclonal antibody complements our portfolio in plant biology and stress research. For your project needs, we additionally offer validated antibody pairs, bulk ancillary reagents, and sourcing of rare targets—all through a single, dependable partner.