Anti-OsELF3 Rabbit Polyclonal Antibody for WB, ELISA - Q9SNQ6

Core Product Specifications and Parameters

Biological Background: OsELF3 Function and Localization

• OsELF3, also known as HD3B, EF7, or ELF3-1, is a rice protein involved in the photoperiodic regulation of flowering time. It acts as a floral promoter by negatively regulating GHD7, a repressor of flowering under both short day (SD) and long day (LD) conditions. Related references: PMID:22399582 PMID:22422935 PMID:22912900
• It functions as a floral activator specifically within the LD photoperiodic pathway. Related references: PMID:22888152
• Under SD conditions, OsELF3 is involved in blue light-induced activation of EHD1 expression, promoting flowering. Related references: PMID:22912900
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Tissue-specific expression is observed in mesophyll cells of young leaves, anthers, stigmas, and the top of lemmas.
• UniProt keywords highlight involvement in flowering, transcription regulation, nucleus localization, and inclusion in the reference proteome.

Experimental Guidance and Technical Tips

• This antibody was generated against an N‑terminal recombinant fragment (amino acids 1–172) of OsELF3. Consider this immunogen design when assessing isoform coverage or potential cross‑reactivity with other ELF3 family members.
• For Western blot (WB) detection, the predicted molecular weight of OsELF3 is approximately 82 kDa. Validate in the relevant rice sample system, and optimize loading controls and transfer conditions accordingly.
• In ELISA applications, the antibody may be used as a capture or detection reagent. Determine optimal coating concentrations and blocking conditions empirically for your assay format.
• Given the role of OsELF3 in flowering time regulation, consider expression profiling across developmental stages or photoperiodic conditions when designing experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials and technical services. For flowering‑time research in rice, we offer this anti‑OsELF3 polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing—supporting your projects from concept to clinic.