Anti-OsEIN2 Polyclonal Antibody for WB, ELISA - Q0D8I9

Core Product Specifications and Parameters

Biological Background: OsEIN2 Function and Localization

• OsEIN2 (ETHYLENE-INSENSITIVE 2), also known as MAO HUZI 7, is a central positive regulator of ethylene signaling in rice (Oryza sativa).
• Acts as a key component in the ethylene signaling pathway, controlling diverse processes such as development, senescence, and grain size.
• OsEIN2 is a membrane protein, predicted to contain transmembrane helices (UniProt keywords: Transmembrane, Transmembrane helix).
• Expressed in various tissues including roots, leaf sheaths, leaf blades, flowers, developing seeds, germinating seeds, young seedlings, vascular tissues, shoot apex, panicle, anthers, pistil, stigma, ovary, seed coat, and fruit coat pericarp. Related references: PMID:15047876
• Classified under the ethylene signaling pathway and reference proteome keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 576-710 of OsEIN2. Validate antibody specificity in the relevant assay system.
• For Western blot (WB), consider the 138 kDa molecular weight: use 6-8% SDS-PAGE and extended transfer times (e.g., overnight at 30V) for efficient transfer of high molecular weight proteins. Include appropriate positive control tissues (e.g., rice seedling or panicle samples).
• For ELISA, optimize coating antigen concentration and detection antibody dilution. A recombinant OsEIN2 fragment or rice tissue extract can serve as coating antigen.
• Polyclonal antibodies may exhibit batch-to-batch variability; initial titration and control experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio streamlines your research and manufacturing supply chain. For ethylene signaling and rice developmental biology studies, we offer validated antibody pairs, target-specific polyclonal antibodies like anti-OsEIN2, bulk ancillary reagents, and custom raw-material sourcing to accelerate your project from concept to clinic.