Anti-OsEIL1 Rabbit Polyclonal Antibody for WB, ELISA - Q10M41

Core Product Specifications and Parameters

Biological Background: OsEIL1 Function and Localization

• Transcription factor serving as a positive regulator in the ethylene response pathway. Related references: PMID:16786297, PMID:25995326, PMID:27701783
• Mediates ethylene-induced inhibition of root growth in etiolated seedlings and directly activates the auxin biosynthetic gene YUCCA8. Related references: PMID:25995326, PMID:28829777
• Functions downstream of EIN2 in disease resistance against rice blast fungus; binds to promoters of NADPH oxidases RBOHA/RBOHB and jasmonate biosynthetic gene OPR4, enhancing ROS generation and phytoalexin accumulation. Related references: PMID:27701783
• Acts as a negative regulator of salt tolerance; during salt stress, activates the HKT1 cation transporter, leading to sodium accumulation in roots. Related references: PMID:25995326
• Binds a specific DNA motif in the GA20OX2 promoter to activate its expression, linking ethylene signaling to gibberellin metabolism. Related references: PMID:30002253
• Subcellular localization: Nucleus.
• Expressed in various tissues including roots, leaf blades, leaf sheaths, stems, internodes, anthers, and developing grains.
• Belongs to the ETHYLENE-INSENSITIVE 3-like (EIL) family; also known as MHZ6.

Experimental Guidance and Technical Tips

• For western blot (WB), consider titrating the antibody starting from a standard dilution and validate using endogenous OsEIL1 expression in appropriate rice tissues, as the target molecular weight is ~71 kDa.
• The immunogen corresponds to a recombinant protein with a C‑terminal peptide sequence (LSRGKDPAGGVVGDPSK); potential cross‑reactivity with other EIL family members should be evaluated through sequence alignment and knockout controls.
• In ELISA applications, the antibody can be used for detection of recombinant or overexpressed OsEIL1; optimize coating antigen concentrations and detection conditions.
• Since the antibody is raised against a rice antigen, performance in other plant species has not been determined; if cross‑species use is intended, verify reactivity experimentally.
• Shipping conditions (ice bag) maintain stability; upon receipt, aliquot and store at recommended temperatures to preserve activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, supporting every phase from concept to clinic. For projects involving plant hormone signaling and stress response, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets—including anti‑OsEIL1 antibodies—to streamline your assay development and procurement needs.