Anti-OsDLT Polyclonal Antibody for WB and ELISA - Q9LWU9

Core Product Specifications and Parameters

Biological Background: OsDLT Function and Localization

• OsDLT, also designated DWARF AND LOW-TILLERING, is a GRAS family transcription factor (OsGRAS-32, SMOS2, D62, GS6) that plays a central role in plant development.
• Acts as a positive regulator of brassinosteroid (BR) signaling, functioning downstream of the receptor BRI1 and the kinase GSK2.
  Related references:
  PMID:19220793
  PMID:22685166
• Direct phosphorylation by GSK2 modulates its activity, linking BR perception to transcriptional responses.
  Related references:
  PMID:22685166
• Mediates feedback inhibition of BR biosynthetic genes and is itself repressed by BZR1.
  Related references:
  PMID:19220793
• Forms a transactivating complex with SMOS1 to induce PHI-1 expression, thereby promoting organ growth.
  Related references:
  PMID:28069545
• The SMOS1–DLT complex serves as an integration point for auxin and BR signaling crosstalk.
  Related references:
  PMID:28069545
• Subcellularly localized to the nucleus, consistent with its role as a transcriptional regulator.
• Broadly expressed in shoot apical meristem, elongating regions, leaf joints, culms, internodes, stems, young panicles, and roots, reflecting its importance in multiple developmental processes.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 1-214 of OsDLT; for Western blot, consider that the full-length protein has a predicted molecular weight of 66 kDa, though post-translational modifications may cause slight shifts.
• For ELISA, optimize coating antigen and antibody concentration; a recombinant protein corresponding to the immunogen region may serve as a positive control.
• Since OsDLT is a nuclear protein, ensure appropriate nuclear extraction protocols when preparing samples from rice tissues for WB.
• Cross-reactivity is expected with Oryza sativa; validate in other plant species if extending the study.
• To study BR signaling, consider co-application with brassinolide or inhibitors like brassinazole in rice seedlings.
• For co-immunoprecipitation or pull-down assays to probe SMOS1 interactions, this antibody may be suitable if the epitope is accessible; further validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For plant biology research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing such as this OsDLT polyclonal antibody. Let us bridge your research needs with reliable, high-quality immunological reagents for brassinosteroid signaling and developmental studies.