Anti-OSBP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P22059

Core Product Specifications and Parameters

Biological Background: OSBP1 Function and Localization

• OSBP1 is a lipid transporter that exchanges sterol with phosphatidylinositol 4-phosphate (PI4P) between the Golgi complex and the endoplasmic reticulum, critical for maintaining cellular lipid homeostasis. Related references: PMID:24209621, PMID:28978670
• It binds cholesterol and oxysterols such as 25-hydroxycholesterol; cholesterol binding promotes the formation of a complex that dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes complex disassembly, implicating OSBP1 in sterol sensing and signaling. Related references: PMID:15746430, PMID:17428193
• OSBP1 regulates cholesterol efflux by modulating the stability of the ABCA1 protein, a key transporter for cholesterol removal from cells. Related references: PMID:18450749
• Localizes to the cytoplasm, perinuclear region, Golgi apparatus, trans-Golgi network, and endoplasmic reticulum membranes, reflecting its role in inter-organelle lipid transport.
• Widely expressed across human tissues.
• Post-translational modifications include acetylation and phosphorylation; contains coiled-coil domains essential for its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 508-807 of human OSBP, a region unique to this protein. Consider using this sequence for Western blot, immunofluorescence, or ELISA as per the validated applications.
• For Western blotting of human, mouse, or rat samples, validate antibody specificity in the relevant cell or tissue lysates, given the reported cross-reactivity.
• In immunofluorescence/ICC, expect perinuclear and cytoplasmic staining patterns consistent with Golgi/ER localization. Validate in a suitable cell line.
• For ELISA, consider using the antibody as a capture or detection reagent; optimize coating and detection conditions.
• As OSBP is involved in cholesterol metabolism, verify antibody performance in models of altered lipid homeostasis if applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For studies on lipid transport and cholesterol metabolism involving OSBP, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Our expertise also extends to sourcing rare-target raw materials, ensuring you have the right tools for every stage of your project.