Anti-OsBip1 Polyclonal Antibody for WB, ELISA - Q6Z7B0

Core Product Specifications and Parameters

Biological Background: OsBip1 Function and Localization

• OsBip1 (Heat shock 70 kDa protein BIP1, also known as Luminal-binding protein 1) is a member of the Hsp70 chaperone family encoded by the BIP1 gene in rice.
• It functions as a molecular chaperone in the endoplasmic reticulum (ER) lumen, facilitating the folding of nascent secretory proteins.
• OsBip1 plays a critical role in ER quality control, ensuring proper folding of seed storage proteins during seed maturation. Related references: PMID:19567376 PMID:21223397
• It acts as a sensor of ER stress, helping to alleviate stress and providing suitable conditions for secretory protein production. Related references: PMID:19567376 PMID:21223397
• The protein is localized to the endoplasmic reticulum lumen.
• Key features include ATP-binding, nucleotide-binding, and glycoprotein modifications, and it is classified under stress response pathways.
• The predicted molecular weight is approximately 73.4 kDa, consistent with the observed band at 73 kDa in rice samples.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-400, a region that may provide specificity for OsBip1 detection; consider using this information when designing ELISA assays or when troubleshooting cross-reactivity.
• For Western blotting, a band around 73 kDa is expected in Oryza sativa samples; validate with a negative control (e.g., knockout or unrelated tissue) to confirm target specificity.
• ELISA optimization may benefit from coating with the recombinant immunogen fragment; include appropriate controls such as pre-immune serum or antigen-blocked wells.
• If cross-reactivity with other species is desired, sequence alignment of the immunogen region is recommended, though the antibody is currently validated only for Oryza sativa.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials and technical support for diagnostic manufacturers and research institutions. For plant stress biology and seed development studies, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. Whether you need rare-target raw-material sourcing or bulk-scale supply, CamelBio is your trusted partner from concept to clinic.