Anti-OsARF19 Polyclonal Antibody for WB, ELISA - Q0D9R7

Core Product Specifications and Parameters

Biological Background: OsARF19 Function and Localization

• OsARF19, also known as OsARF7a, is an auxin response factor (ARF) that functions as a transcriptional regulator in rice (Oryza sativa).
• It binds specifically to the auxin-responsive promoter element (AuxRE) with the consensus DNA sequence 5'-TGTCTC-3', mediating auxin-dependent gene expression.
• The protein contains a DNA-binding domain that enables direct interaction with target gene promoters, linking auxin signaling to transcriptional responses.
• OsARF19 localizes to the nucleus, consistent with its role as a transcription factor.
• It is expressed in multiple tissues including roots, culms, leaves, and young panicles, indicating a broad regulatory role in rice development.
• The gene undergoes alternative splicing, potentially generating multiple isoforms with distinct regulatory properties.
• Keywords associated with OsARF19 include alternative splicing, auxin signaling pathway, DNA-binding, nucleus, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein corresponding to amino acids 800-900 of OsARF19 (UniProt Q0D9R7). This region may confer specificity; researchers should validate reactivity in their sample system.
• For Western blot (WB), the predicted molecular weight is ~128 kDa. Use appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• ELISA applications may require optimization of coating antigen and antibody dilution. Consider using the recombinant protein immunogen as a positive control.
• As a polyclonal antibody, it may recognize multiple epitopes and potentially different isoforms resulting from alternative splicing. Confirm specificity using knockout or knockdown controls if available.
• Cross-reactivity is reported for Oryza sativa; when working with other plant species, test for cross-reactivity before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with critical reagents. This anti-OsARF19 polyclonal antibody supports plant biology research, particularly in auxin signaling pathways. Beyond individual antibodies, CamelBio facilitates procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare target raw materials—helping you streamline your supply chain from early-stage development to scaled production.