Anti-OsALDH2B1 Polyclonal Antibody for WB, ELISA - Q9FRX7

Core Product Specifications and Parameters

Biological Background: OsALDH2B1 Function and Localization

• OsALDH2B1 (Aldh2b) is a protein encoded by the Aldh2b gene in rice (Oryza sativa subsp. japonica), identified through proteomics approaches.
• Based on sequence homology, OsALDH2B1 belongs to the aldehyde dehydrogenase (ALDH) superfamily, which typically catalyzes the NAD+-dependent oxidation of aldehydes.
• The protein features conserved ALDH domains and is annotated as an oxidoreductase, suggesting a role in metabolic aldehyde detoxification.
• Keywords from the UniProt record (Q9FRX7) include NAD, oxidoreductase, and proteomics identification, indicating its cofactor preference and experimental detection.
• Detailed functional studies and subcellular localization data are currently unavailable for this entry (UniProtKB unreviewed, TrEMBL), but homologous ALDHs are often mitochondrial or cytosolic.
• The predicted molecular weight of the unprocessed precursor is approximately 59.3 kDa, consistent with the observed ~59 kDa band on SDS-PAGE.
• Tissue-specific expression and biological functions in rice development or stress responses remain to be elucidated.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-100) of OsALDH2B1, so the antibody is likely to detect the target protein in denaturing Western blot conditions.
• For WB, consider using a standard SDS-PAGE protocol and validate the antibody with rice tissue lysates expressing endogenous ALDH2B1.
• In ELISA, optimize coating antigen (recombinant protein or peptide) concentration and antibody dilution by checkerboard titration.
• Cross-reactivity is specified for Oryza sativa; testing on other species or closely related ALDH isoforms is recommended if broader reactivity is desired.
• Due to the TrEMBL status of Q9FRX7, we recommend verifying the target identity by mass spectrometry or immunoprecipitation in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material provider, CamelBio offers comprehensive access to high-quality antibodies, including this OsALDH2B1 polyclonal antibody, supporting plant biology and agricultural research applications. Beyond this product, our one-stop platform enables you to source validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target development for diagnostic and research workflows. Partnering with CamelBio streamlines your procurement process from concept to clinic, ensuring reliable supply of critical raw materials for your aldehyde dehydrogenase studies or broader enzyme research needs.