Anti-ORP150/GRP170/HSP12A Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y4L1

Core Product Specifications and Parameters

Biological Background: ORP150/GRP170/HSP12A Function and Localization

• Hypoxia up-regulated protein 1 (HYOU1), also known as ORP-150, GRP-170, and heat shock protein family H member 4, is a molecular chaperone.
• It plays a pivotal role in cytoprotective mechanisms triggered by oxygen deprivation; promotes HSPA5/BiP-mediated ATP nucleotide exchange and activates the unfolded protein response (UPR) pathway under endoplasmic reticulum (ER) stress (By similarity).
• The protein localizes to the endoplasmic reticulum lumen.
• HYOU1 is highly expressed in tissues with extensive ER and high secretory protein synthesis, such as liver and pancreas; lower expression is found in brain and kidney. It is also present in macrophages within aortic atherosclerotic plaques and in breast cancers.
• The protein contains ATP-binding and nucleotide-binding domains, characteristic of heat shock proteins.
• Post-translational modifications include acetylation, glycosylation, and phosphorylation, as indicated by keywords.
• Alternative splicing and disease variants have been reported for this gene.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 111 kDa. Consider using positive control lysates from liver or pancreas, which express high levels of HYOU1.
• For immunohistochemistry (IHC-P), validate tissue fixation and antigen retrieval protocols, as the target is located in the ER lumen. Include positive tissue controls such as liver or atherosclerotic plaque sections.
• For ELISA, we recommend titrating the antibody as a capture or detection reagent and validating performance with recombinant protein or cell lysates.
• The immunogen spans the N-terminal region (aa 1-150), so the antibody may recognize variants containing this epitope. Validate specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for hypoxia and ER stress targets. We also support custom sourcing for rare targets and offer technical consulting to accelerate your assay development from concept to clinic.