Anti-ORP1 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9BXW6

Core Product Specifications and Parameters

Biological Background: ORP1 Function and Localization

• ORP1, encoded by the OSBPL1A gene, is an oxysterol-binding protein-related protein, also known as OSBP8 or OSBPL1B. It features ankyrin repeats and a coiled-coil domain, with a molecular weight of approximately 108 kDa.
• The protein specifically localizes to late endosomes, where it plays a critical role in lipid transport and endosomal function.
• ORP1 binds phospholipids, exhibiting strong affinity for phosphatidic acid and weaker affinity for phosphatidylinositol 3-phosphate (By similarity).
• It stabilizes the GTP-bound form of RAB7A on late endosomes/lysosomes, thereby modulating late endocytic compartment functionality. Related references: PMID:16176980
• ORP1 binds 25-hydroxycholesterol and cholesterol, suggesting involvement in cholesterol sensing and intracellular transport. Related references: PMID:17428193
• Alternative splicing generates multiple isoforms, and the protein is subject to phosphorylation, as identified in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 851–950). Verify antibody specificity through knockdown or knockout controls in the relevant sample system.
• For western blotting, expect a band at ~108 kDa. Detection should be validated in human, mouse, and rat lysates as per cross-reactivity data.
• For immunofluorescence or immunocytochemistry, optimize fixation and permeabilization conditions to effectively access late endosomal targets.
• For ELISA, the antibody can be considered as a capture or detection reagent; empirical pairing with other ORP1 antibodies is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. With expertise in lipid metabolism and endosomal trafficking targets like ORP1, CamelBio supports diagnostic manufacturers, labs, and research institutes from concept to clinic, including rare target raw-material sourcing.