Anti-ORMDL3 Rabbit Polyclonal Antibody for WB and ELISA - Q8N138

Core Product Specifications and Parameters

Biological Background: ORMDL3 Function and Localization

• ORMDL3 (ORM1-like protein 3) is a member of the ORMDL family and exists in multiple isoforms due to alternative splicing.
• It is a key regulator of sphingolipid de novo biosynthesis, modulating serine palmitoyltransferase (SPT) activity in response to ceramide levels. Related references: PMID:20182505 PMID:30700557 PMID:37308477
• Ceramide binding to ORMDL3's N-terminus stabilizes a conformation that blocks SPT substrate entry, preventing ceramide accumulation and apoptosis.
• The protein localizes to the endoplasmic reticulum membrane.
• It is widely expressed in tissues including heart, brain, lung, liver, skeletal muscle, kidney, pancreas, placenta, and fetal spleen and thymus.
• Post-translational modifications include hydroxylation and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1–100); consider this when selecting detection or blocking peptides.
• For Western blotting, a band of approximately 17 kDa is expected under reducing conditions; validate in human and mouse lysates.
• In ELISA, the antibody may be used for direct or sandwich assays; titrate and optimize with the relevant recombinant protein or peptide.
• Due to the target's ER membrane residence, consider appropriate lysis and sample preparation protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies like this anti-ORMDL3 polyclonal antibody alongside a broad portfolio of recombinant proteins, antigen peptides, and ancillary reagents. Whether for sphingolipid metabolism research or clinical diagnostic development, we provide reliable supply chain solutions from discovery to scale-up. Contact us to discuss customized antibody development and bulk ordering.