Anti-ORM1/2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P02763 / P19652

Core Product Specifications and Parameters

Biological Background: ORM1/2 Function and Localization

• ORM1 encodes Alpha-1-acid glycoprotein 1 (AGP1), also known as Orosomucoid-1, a major acute-phase plasma protein secreted by the liver.
• ORM2 (AGP2) is a closely related isoform; this antibody recognizes both ORM1 and ORM2 due to sequence homology in the N-terminal immunogen region.
• Functions as a transport protein in the bloodstream, binding various endogenous and exogenous ligands within its beta-barrel structure.
• Modulates immune system activity during the acute-phase response, influencing inflammatory processes.
• Binds and transports synthetic drugs, affecting their distribution and bioavailability in the body.
• Subcellular localization is primarily extracellular (secreted into plasma); tissue specificity is hepatic expression.
• Post-translational modifications include glycosylation, leading to an apparent molecular weight of approximately 47 kDa in SDS-PAGE, higher than the calculated 23.5 kDa.
• Keywords: Acute phase, Glycoprotein, Secreted, Transport; structurally features disulfide bonds and pyrrolidone carboxylic acid.

Experimental Guidance and Technical Tips

• For Western blot, the predicted band may appear around 47 kDa due to extensive glycosylation; validate in relevant human plasma or liver tissue lysates.
• IHC-P: Consider antigen retrieval methods (e.g., heat-induced epitope retrieval) and appropriate positive controls such as human liver sections.
• ELISA: The antibody can be used as a capture or detection reagent; pairing with a validated polyclonal detection antibody may enhance sensitivity.
• Since the immunogen is a synthetic peptide from the N-terminus, ensure the epitope is accessible in denaturing and native conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For researchers studying acute-phase proteins, inflammation, or drug transport, we provide reliable access to high-quality anti-ORM1/2 reagents and related consumables to accelerate your diagnostic development from concept to clinic.