Anti-Orexin B/Hypocretin-2 Rabbit Polyclonal Antibody for WB, ELISA - O43612

Core Product Specifications and Parameters

Biological Background: Orexin B/Hypocretin-2 Function and Localization

• The hypocretin neuropeptide precursor (prepro-orexin) is processed into two mature neuropeptides: orexin-A (hypocretin-1) and orexin-B (hypocretin-2), which share sequence homology but have distinct receptor binding profiles.
• Orexin-B/Hypocretin-2 binds specifically to the orexin receptor HCRTR2/OX2R and plays key roles in the central regulation of feeding behavior, energy homeostasis, and sleep-wake cycles.
• Orexinergic neurons are primarily localized in the lateral hypothalamus; at the subcellular level, the precursor localizes to the rough endoplasmic reticulum, cytoplasmic vesicles, and synapses.
• Orexin-B/Hypocretin-2 is involved in modulating pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner, suggesting a role in neuroendocrine reproductive function.
• The precursor contains a signal peptide, undergoes cleavage on pairs of basic residues, and features post-translational modifications including amidation and pyrrolidone carboxylic acid formation, which are critical for bioactivity.
• Abundant expression is detected in the subthalamic nucleus, while other brain regions and peripheral tissues show no detectable expression (hypothalamus was not tested).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human Orexin B/Hypocretin-2. The antibody may recognize both the precursor and the mature peptide depending on sample preparation and processing.
• For Western blot (WB), consider the predicted precursor molecular weight of ~13 kDa and the smaller mature orexin-B peptide; validate detection using relevant positive controls such as brain tissue lysates or recombinant protein.
• In ELISA, evaluate direct peptide coating or sandwich formats; assess potential cross-reactivity with orexin-A due to sequence similarity within the immunogen region.
• Include appropriate positive and negative controls, and optimize dilution factors empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your neuropeptide and metabolic research needs. Let us help streamline your procurement for orexin-related assay development.