Anti-ORC6 Polyclonal Antibody for WB, ELISA - Q9Y5N6

Core Product Specifications and Parameters

Biological Background: ORC6 Function and Localization

• ORC6 is a subunit of the origin recognition complex (ORC), which binds to origins of replication to initiate DNA replication.
• DNA-binding is ATP-dependent; the specific DNA sequences defining origins of replication have not been identified yet.
• ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
• ORC6 does not bind histone H3 and H4 trimethylation marks H3K9me3, H3K27me3, and H4K20me3.
• Subcellular localization is nuclear, consistent with its role in DNA replication.
• Mutations in ORC6 are associated with Meier-Gorlin syndrome, a form of primordial dwarfism (keywords: Disease variant, Dwarfism).

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 252 amino acids; consider this region for epitope mapping and competition assays.
• For Western blot, ORC6 migrates at approximately 28 kDa; validate band specificity using knockout or knockdown controls in human, mouse, or rat cell lines.
• In ELISA, use the recombinant immunogen as a positive control and consider testing against native ORC complex in nuclear extracts.
• Cross-reactivity with mouse and rat is predicted based on sequence homology; experimental validation in the relevant species is recommended.
• For DNA replication studies, synchronize cells to enrich for the pre-replication complex and enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents to support DNA replication and cell cycle research. We supply optimized monoclonal and polyclonal antibodies, bulk reagents, and can assist with rare target raw-material sourcing, ensuring seamless procurement from concept to clinic. Contact us to discuss how our ORC6 antibody and comprehensive supply capabilities can accelerate your diagnostic development.