Anti-ORC2 Polyclonal Antibody for WB, ELISA - Q13416

Core Product Specifications and Parameters

Biological Background: ORC2 Function and Localization

• ORC2 (Origin Recognition Complex Subunit 2) is a critical component of the origin recognition complex (ORC), which binds to origins of replication in an ATP-dependent manner, although specific DNA sequences defining origins in humans remain unidentified.
• The ORC complex, including ORC2, is essential for the assembly of the pre-replication complex, a prerequisite for initiating DNA replication.
• ORC2 contains a bromo-adjacent homology (BAH) domain that mediates binding to histone H3 and H4 trimethylation marks, specifically H3K9me3, H3K20me3, and H4K27me3, linking DNA replication initiation to chromatin status.
• It plays a role in protein stabilization by protecting LRWD1 from ubiquitin-mediated proteasomal degradation and also contributes to the stabilization of ORC3.
• ORC2 is encoded by the ORC2 gene (also known as ORC2L) and localizes to the nucleus, consistent with its function in DNA replication.
• The protein is subject to phosphorylation and has a theoretical molecular weight of approximately 66 kDa, aligning with the observed band in Western blot analysis.
• Keywords from UniProt include DNA replication, nucleus, phosphoprotein, and reference proteome, highlighting its conserved and essential role in cell cycle progression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 458-577 near the C-terminus of human ORC2, suggesting the antibody may recognize full-length ORC2; consider verifying specificity using lysates with known ORC2 expression.
• For Western blot, a band around 66 kDa is expected. Use appropriate positive controls such as human cell lines (e.g., HeLa, HEK293) and validate in the relevant sample system.
• This antibody is suitable for ELISA; assay conditions should be optimized for the specific protocol and target.
• Cross-reactivity has been confirmed for human ORC2; reactivity with other species has not been tested, so validation is recommended if used beyond human samples.

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CamelBio offers a comprehensive sourcing solution for IVD raw materials, connecting diagnostic manufacturers and research labs with validated antibodies like this anti-ORC2 polyclonal antibody for DNA replication and chromatin research. Our portfolio includes optimized polyclonal antibodies, ancillary reagents, and rare target sourcing to support your assay development and production needs. Partner with CamelBio for streamlined access to high-quality raw materials from concept to clinic.