Anti-ORAI1 Rabbit Polyclonal Antibody for WB and ELISA - Q96D31

Core Product Specifications and Parameters

Biological Background: ORAI1 Function and Localization

• ORAI1 is a pore-forming subunit of store-operated CRAC channels, which mediate Ca2+ influx upon ER Ca2+ store depletion and activation by the Ca2+ sensor STIM1. These channels are the main pathway for Ca2+ entry in T cells and play a critical role in immune responses. Related references: PMID:16645049 PMID:16733527 PMID:16807233

• ORAI1 assembles with ORAI2 and ORAI3 to form hexameric CRAC channels with distinct calcium dynamics. ORAI1 mainly contributes to sustained Ca2+ plateaus, while ORAI2 and ORAI3 generate oscillatory patterns, enabling cell-type specific signaling. Related references: PMID:16645049 PMID:16733527 PMID:16807233

• CRAC channel-mediated Ca2+ influx is coupled to NFAT transcription factors via AKAP5-organized signaling microdomains, leading to NFATC2/NFAT1 and NFATC3/NFAT4 activation and cytokine transcription essential for adaptive immunity. Related references: PMID:16582901 PMID:17442569 PMID:19182790

• ORAI1 also forms ARC and LRC channels with ORAI3, mediating store-independent Ca2+ influx in response to arachidonic acid or leukotriene C4, highlighting its role in inflammatory signaling. Related references: PMID:19622606 PMID:32415068

• At the basolateral membrane of mammary epithelial cells, ORAI1 facilitates transepithelial Ca2+ transport for milk secretion, operating independently of ER/Golgi Ca2+ stores. Related references: PMID:20887894

• In humans, ORAI1 protein is detected in naive CD4+ and CD8+ T cells, with similar expression levels in naive and effector T helper cells, underscoring its role in T cell biology.

• The protein undergoes alternative initiation, glycosylation, phosphorylation, and ubiquitination, and contains a calmodulin-binding domain, as indicated by UniProt keywords, suggesting complex post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human ORAI1; this antibody may preferentially detect the full-length protein and N-terminal variants.
• For Western blot (WB), consider using whole cell lysates from ORAI1-expressing cell lines (e.g., Jurkat T cells) or tissues with high ORAI1 expression; the predicted molecular weight is approximately 33 kDa.
• For ELISA, validate the antibody using recombinant ORAI1 protein or the immunogenic peptide as a standard, and optimize coating and detection conditions.
• Cross-reactivity with mouse and rat ORAI1 is reported; verify sequence homology and test in your relevant species applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material sourcing solutions, including this anti-ORAI1 polyclonal antibody for calcium signaling and immune research. As a one-stop platform, we provide reliable access to a wide range of antibodies, antigens, and ancillary reagents to support your assay development needs. Whether you require validated antibody pairs, bulk quantities, or assistance with rare target sourcing, CamelBio is your partner from concept to clinic.