Anti-OR10H3 Polyclonal Antibody for WB, IHC-P, ELISA - O60404

Core Product Specifications and Parameters

Biological Background: OR10H3 Function and Localization

• OR10H3, also designated olfactory receptor OR19-24, is a member of the olfactory receptor gene family, the largest G protein-coupled receptor (GPCR) family in mammals.
• It functions as an odorant receptor, responsible for detecting odorant molecules and initiating the perception of smell by activating olfactory signal transduction pathways.
• The receptor is localized to the cell membrane and features a characteristic seven-transmembrane domain structure typical of GPCRs.
• OR10H3 contains conserved structural motifs, including disulfide bonds, that are important for receptor folding, stability, and ligand-binding activity.
• As part of the reference proteome, its protein existence is supported by experimental evidence at the protein level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100), which is extracellular and may provide good epitope accessibility in native and denatured conditions.
• For Western blot, a band near 36 kDa is predicted; however, due to the hydrophobic nature of GPCRs, running conditions and sample preparation may require optimization (e.g., mild denaturation at 37°C instead of boiling).
• In IHC-P, consider using olfactory epithelium or other appropriate tissue sections as positive controls, and perform antigen retrieval as needed for membrane proteins.
• For ELISA, the immunizing peptide can serve as a positive coating antigen to verify antibody reactivity and titer.

CamelBio: Your One-Stop Sourcing Bridge

As a leading one-stop IVD raw-material partner, CamelBio offers diagnostic manufacturers and researchers validated antibody pairs, optimized polyclonal antibodies like this anti-OR10H3, and bulk ancillary reagents. Whether you are developing olfactory-based diagnostic tools or sourcing rare GPCR antibodies, our comprehensive supply chain ensures reliable, scalable access from concept to clinical application.