Anti-OPTN Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q96CV9

Core Product Specifications and Parameters

Biological Background: OPTN Function and Localization

• Optineurin (OPTN), also known as FIP-2, HIP-7, or NRP, is a ~66 kDa multidomain protein containing zinc finger and coiled-coil motifs.
• Plays a critical role in Golgi maintenance and membrane trafficking by linking myosin VI to the Golgi, essential for Golgi ribbon formation. Related references: PMID:27534431
• Functions in innate immunity: recruits TBK1 to the Golgi upon viral infection, leading to IRF3 phosphorylation and IFN-beta production. Related references: PMID:27538435
• Acts as a selective autophagy receptor, targeting ubiquitinated bacteria (e.g., Salmonella) for degradation via LC3-interaction, cooperating with SQSTM1 and CALCOCO2/NDP52.
• In viral infections, can be hijacked by adenovirus E3 14.7 and Bluetongue virus to suppress innate immune signaling. Related references: PMID:27538435 PMID:9488477
• Localizes to the cytoplasm, perinuclear region, Golgi, trans-Golgi network, autophagosomes, and recycling endosomes.
• Highly expressed in skeletal muscle, heart, brain, and eye tissues (trabecular meshwork, aqueous humor).
• Disease relevance: Mutations in OPTN cause amyotrophic lateral sclerosis type 12 (ALS12) and primary open-angle glaucoma (GLC1E).

Experimental Guidance and Technical Tips

• The full-length immunogen (aa 1-577) detects endogenous OPTN across multiple assays. For Western blot, a predominant band around 66 kDa is expected; include appropriate positive and negative controls to confirm specificity.
• For immunohistochemistry (paraffin-embedded sections, IHC-P), consider optimizing antigen retrieval and signal detection; validate staining in tissues known to express OPTN, such as human skeletal muscle or mouse brain.
• This antibody is compatible with immunoprecipitation (IP) and ELISA; for IP, use a starting amount of 1-5 µg per 500 µg lysate and corroborate with target-specific co-IPs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material procurement, offering validated antibody reagents like this anti-OPTN polyclonal antibody along with complementary tools for autophagy, neurodegeneration, and innate immunity research. We provide a wide range of bulk ancillary reagents, optimized monoclonal/polyclonal antibodies, and custom sourcing for rare targets, ensuring a seamless supply chain from development to clinical application.