Anti-OPRD1 Rabbit Polyclonal Antibody for WB, ELISA - P41143

Core Product Specifications and Parameters

Biological Background: Delta-type Opioid Receptor Function and Localization

• The delta-type opioid receptor (OPRD1) is a G protein-coupled receptor (GPCR) that serves as the primary receptor for endogenous enkephalins and other opioids.
• Ligand binding triggers G protein-mediated signaling, inhibiting adenylate cyclase activity, which reduces cAMP levels and modulates neuronal excitability.
• OPRD1 signaling decreases calcium ion currents and increases potassium ion conductance, leading to inhibited neurotransmitter release.
• This receptor plays a critical role in the perception of pain and mediates opiate-induced analgesia.
• OPRD1 is implicated in the development of analgesic tolerance to morphine, a key consideration in pain management research.
• Subcellularly, OPRD1 is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• Post-translational modifications include glycosylation, palmitoylation, and disulfide bond formation, which are typical for GPCR maturation and function.
• Tissue expression is prominent in brain regions such as the cerebral cortex, hypothalamus, hippocampus, and olfactory bulb, and it is also detected in oocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (aa 300-372); the antibody may recognize both unmodified and post-translationally modified OPRD1. Validate for your specific experimental conditions.
• For Western blot (WB), the predicted molecular weight is approximately 40 kDa; however, glycosylation may cause an upward shift. Consider using deglycosylation enzymes if necessary.
• OPRD1 expression is highest in neural tissues (brain cortex, hypothalamus); include appropriate positive controls from these tissues.
• The antibody has been tested for ELISA; for other applications like IHC or IF, optimization may be required.
• Cross-reactivity with mouse and rat is noted, making it suitable for studies in these model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive suite of IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-OPRD1 polyclonal antibody is part of our extensive portfolio for neuroscience and pain research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic and research needs.