Anti-OPRD1 Rabbit Monoclonal Antibody for WB, ELISA - P41143

Core Product Specifications and Parameters

Biological Background: OPRD1 Function and Localization

• OPRD1 encodes the delta-type opioid receptor, a G protein-coupled receptor (GPCR) that belongs to the opioid receptor family.
• Upon binding endogenous enkephalins or exogenous opioids, it triggers G protein signaling, primarily inhibiting adenylate cyclase activity and reducing cAMP levels.
• This receptor also modulates ion channels: it reduces calcium ion currents and increases potassium ion conductance, leading to inhibition of neurotransmitter release.
• OPRD1 is critically involved in the perception of pain and mediates opioid-induced analgesia; it also plays a role in the development of analgesic tolerance to morphine.
• The protein is localized at the cell membrane, consistent with its function as a transmembrane receptor.
• It is expressed in various brain regions including the cortex, hypothalamus, hippocampus, and olfactory bulb, and has been detected in oocytes.
• Post-translational modifications include glycosylation, palmitoylation, and disulfide bond formation, which are critical for receptor folding and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100), which may be suitable for detecting both unmodified and certain post-translationally modified forms of OPRD1; however, epitope accessibility should be validated in the relevant sample system.
• For Western blot, a band of approximately 40 kDa is expected; use appropriate cell or tissue lysates known to express OPRD1, such as brain tissue or neuronal cell lines.
• This antibody can be employed in ELISA as either a capture or detection antibody; consider pairing with a validated antibody pair for optimal sandwich ELISA development.
• Given the reported cross-reactivity with mouse and rat, verify specificity in your species of interest, especially in brain tissue where homologous opioid receptors are expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for diagnostic manufacturers and research laboratories, providing rapid access to validated IVD raw materials including monoclonal antibodies like this anti-OPRD1 clone. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio streamlines the supply chain from concept to clinic. Our portfolio supports diverse research areas such as pain biology and GPCR signaling.