Anti-OPN5 Rabbit Polyclonal Antibody for WB, ELISA - Q6U736

Core Product Specifications and Parameters

Biological Background: OPN5 Function and Localization

• OPN5, also known as neuropsin, GPR136, or TMEM13, is a G protein-coupled opsin receptor that functions as a photoreceptor in the retina and other tissues.
• It acts as a G protein-coupled photoreceptor that selectively activates G(i) protein in response to light. Activation involves light-induced isomerization of the opsin-bound cis-retinal to all-trans-retinal, triggering a phototransduction cascade. The protein is bistable, absorbing UVA (380 nm) and blue (470 nm) light for photoreversible regeneration. Related references: PMID:22043319
• Required for light response in the inner plexiform layer and contributes to regulation in the nerve fiber layer via phosphorylated DAT/SLC6A3 dopamine uptake modulation.
• Involved in local corneal and retinal circadian rhythm photoentrainment by modulating UVA light-induced phase-shift of the retina clock. Acts as a circadian photoreceptor in the outer ear and vibrissal pads, regulating clock-gene expression in response to violet light.
• Plays a role in postnatal retinal development by negatively regulating hyaloid vessel regression through light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling.
• Mediates violet light-accelerated corneal wound healing, being transiently expressed after physical injury.
• Localizes to the cell membrane and is detected in the brain, retina, and neural retina-derived cell lines.
• Contains conserved features of G protein-coupled receptors, including transmembrane domains, disulfide bonds, and palmitoylation sites, and belongs to the opsin family of photoreceptor proteins.

Experimental Guidance and Technical Tips

• This antibody was generated against a synthetic peptide corresponding to amino acids 200-300 of human OPN5. Validate specificity in your target species (human, mouse, rat) by using appropriate positive and negative controls.
• For western blotting, the predicted molecular weight of OPN5 is approximately 40 kDa (UniProt: 39.7 kDa). Ensure proper electrophoresis conditions and confirm band identity with lysates from OPN5-expressing tissues such as retina or neural retina-derived cell lines.
• ELISA-based detection may require optimization of coating antigen concentration and antibody titer. Consider using recombinant OPN5 or peptide as coating antigen.
• Cross-reactivity with mouse and rat OPN5 is supported by sequence homology; however, performance in these species should be independently validated.
• As a polyclonal antibody, lot-to-lot consistency should be monitored in critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and researchers with a comprehensive portfolio of raw materials, including this anti-OPN5 polyclonal antibody for photoreception and circadian rhythm studies. We offer validated antibody pairs, optimized antibodies, and bulk ancillary reagents, along with rare target sourcing to accelerate your diagnostic or research development. Our team provides one-stop access to high-quality raw materials and technical services from concept to clinic.