Anti-OPLAH Rabbit Polyclonal Antibody for WB and ELISA - O14841

Core Product Specifications and Parameters

Biological Background: OPLAH Function and Localization

• OPLAH (5-oxoprolinase) is a hydrolase that catalyzes the ATP-dependent cleavage of 5-oxo-L-proline to L-glutamate, playing a key role in the glutathione degradation pathway and glutamate metabolism.
• The enzyme is also known as 5-OPase or pyroglutamase, with official alternative names OPLA and OPLAHD.
• OPLAH localizes to the cytoplasm, specifically the cytosol, where it processes its substrate.
• As an ATP-binding protein, it couples nucleotide hydrolysis to its enzymatic activity, classifying it as a nucleotide-binding hydrolase.
• Multiple phosphoproteomic studies have identified OPLAH as a phosphoprotein, indicating potential post-translational regulation.
• UniProt annotations highlight its function as a hydrolase, ATP-binding, and cytoplasmic localization; it is part of the reference proteome and has been widely detected in proteomics screens.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1119–1288 in the C-terminal region of human OPLAH, which may provide specificity for the full-length protein; cross-reactivity with mouse and rat orthologs should be verified experimentally.
• For western blotting (WB), the predicted molecular weight is 137 kDa; use appropriate high-molecular-weight markers and validate in the endogenous expression system of interest.
• In ELISA applications, consider using this polyclonal antibody as a capture or detection reagent following standard protocol optimization.
• Because OPLAH is a cytoplasmic enzyme, prepare total cell or cytosolic extracts for WB, and confirm expression levels in relevant tissues (e.g., liver, kidney) based on target function.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-OPLAH product, and ancillary reagents. We support projects focused on glutamate metabolism and related pathways by offering one-stop access to high-quality raw materials, technical consultation, and supply chain solutions. Whether you need bulk quantities or rare target sourcing, CamelBio accelerates your development from concept to clinic.