Anti-OPCML Polyclonal Antibody for WB, ELISA - Q14982

Core Product Specifications and Parameters

Biological Background: OPCML Function and Localization

• OPCML is an opioid-binding protein that, in the presence of acidic lipids, interacts with opioids, suggesting a role in modulating opioid signaling.
• It functions as a cell adhesion molecule, probably mediating cell-contact interactions.
• Belongs to the IgLON family (immunoglobulin LON superfamily), characterized by immunoglobulin domains and GPI anchorage.
• Localizes to the cell membrane, anchored via a glycosylphosphatidylinositol (GPI) moiety.
• Undergoes alternative splicing, generating multiple isoforms; annotated with keywords including glycoprotein, disulfide bond, and signal peptide.
• Implicated in human disease variants, highlighting its potential clinical relevance.
• The calculated molecular weight of the canonical isoform is approximately 38 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 246–345 of human OPCML (NP_002536.1), spanning a region likely within the extracellular domain; consider verifying its conservation across mouse and rat for predicted cross-reactivity.
• For Western blotting (WB), typical molecular weight detection is ~38 kDa; include positive and negative controls from relevant tissues or cell lines, and optimize protein loading to account for potential glycosylation-induced band shifts.
• For ELISA, assess the coating antigen concentration and antibody titer in the specific assay format; consider using recombinant OPCML protein or cell lysates.
• Due to the polyclonal nature of the antibody, lot-to-lot variability may exist; it is advisable to validate each new batch in the intended application.
• If performing immunofluorescence or other cell-based assays, note that OPCML is membrane-anchored; appropriate permeabilization may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-OPCML antibody supports neuroscience and cell adhesion research, and CamelBio's sourcing capabilities extend to rare targets and custom reagent solutions to accelerate your assay development pipeline.