Anti-OPA3 Polyclonal Antibody for WB, ELISA - Q9H6K4

Core Product Specifications and Parameters

Biological Background: OPA3 Function and Localization

• OPA3 (Optic atrophy 3 protein) is a ubiquitously expressed mitochondrial protein encoded by the OPA3 gene, with prominent expression in skeletal muscle and kidney.
• It may play a role in mitochondrial processes, though its exact molecular function remains under investigation.
• OPA3 localizes predominantly to mitochondria, consistent with its potential involvement in mitochondrial dynamics or metabolism.
• The OPA3 gene undergoes alternative splicing, generating multiple isoforms.
• Mutations in OPA3 are associated with autosomal dominant optic atrophy and cataract, highlighting its importance in vision and sensory transduction.
• The protein features a coiled-coil domain, suggesting possible protein-protein interactions.
• It has a predicted molecular weight of approximately 20 kDa.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~20 kDa; consider using a 12-15% SDS-PAGE gel and appropriate transfer conditions.
• The immunogen covers amino acids 25-179, so the antibody may recognize multiple isoforms if the epitope is conserved.
• Validate cross-reactivity in mouse and rat samples using appropriate positive controls.
• For ELISA, follow standard direct or indirect protocols and optimize coating antigen concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-OPA3 polyclonal antibody supports studies in mitochondrial biology, optic atrophy, and vision research, with our team ready to assist in sourcing complementary reagents or custom solutions for your project.