Anti-OPA1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O60313

Core Product Specifications and Parameters

Biological Background: OPA1 Function and Localization

• OPA1 (Optic atrophy protein 1) is a dynamin-like GTPase that exists as two main forms: a long transmembrane form (L-OPA1) tethered to the inner mitochondrial membrane, and a short soluble form (S-OPA1) in the intermembrane space, generated by OMA1 cleavage (PubMed:31922487, PubMed:32228866, PubMed:33237841). Related references: PMID:31922487, PMID:32228866, PMID:33237841
• OPA1 is a key mediator of mitochondrial inner membrane fusion and cristae morphology. L-OPA1 and S-OPA1 cooperate to form helical assemblies that drive membrane curvature and fusion via GTP hydrolysis (PubMed:28628083, PubMed:37612504, PubMed:37612506). Related references: PMID:28628083, PMID:37612504, PMID:37612506
• Belongs to the dynamin superfamily of large GTPases, characterized by conserved domains involved in self-assembly and membrane remodeling.
• Subcellular location: Mitochondrion inner membrane and intermembrane space.
• Essential for mitochondrial genome maintenance (PubMed:18158317, PubMed:20974897) and regulation of apoptosis through cristae remodeling and cytochrome c release. Related references: PMID:18158317, PMID:20974897
• Highly expressed in retina, brain, testis, heart, and skeletal muscle (PubMed:11017079, PubMed:11017080, PubMed:11810270). Related references: PMID:11017079, PMID:11017080, PMID:11810270
• Post-translational modifications: Proteolytic processing by PARL and OMA1 generates S-OPA1, influencing mitochondrial dynamics. Acetylation and disulfide bond formation are also noted.
• Disease relevance: Mutations in OPA1 cause autosomal dominant optic atrophy, and are associated with mitochondrial syndromes, deafness, and cardiomyopathy.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight of OPA1 is approximately 112 kDa; multiple isoforms may be detected due to alternative splicing and proteolytic processing. Consider loading sufficient protein and using gradient gels for resolution.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), OPA1 localizes to mitochondria, showing a characteristic punctate pattern. Mitochondrial co-staining is recommended for verification.
• The immunogen corresponds to amino acids 200-600 of human OPA1, an internal region conserved across isoforms; thus, the antibody may recognize multiple OPA1 variants. Validate in the relevant species and sample system.
• As a polyclonal antibody, lot-to-lot variability may occur; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner for diagnostic manufacturers, labs, and research institutes. This anti-OPA1 polyclonal antibody supports research into mitochondrial dynamics, apoptosis, and optic atrophy. Beyond single antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, helping to streamline your assay development from concept to clinic.