Anti-OPA1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IF-P, ELISA - O60313

Core Product Specifications and Parameters

Biological Background: OPA1 Function and Localization

• OPA1 is a dynamin-related GTPase essential for normal mitochondrial morphology. It exists as two major forms: a long transmembrane form (L-OPA1) anchored to the inner mitochondrial membrane and a short soluble form (S-OPA1) generated by proteolytic cleavage in the intermembrane space.
  Related references: PMID:31922487, PMID:32228866, PMID:33237841
• L-OPA1 and S-OPA1 cooperate to mediate mitochondrial inner membrane fusion, a process critical for mitochondrial network maintenance and respiratory chain function.
  Related references: PMID:16778770, PMID:17709429, PMID:20185555, PMID:31922487, PMID:37612504, PMID:37612506
• The equilibrium between L-OPA1 and S-OPA1 is tightly regulated: excessive S-OPA1 production by OMA1 following mitochondrial depolarization disrupts fusion and alters cristae morphology.
  Related references: PMID:20038677, PMID:31922487
• OPA1 plays a role in mitochondrial genome maintenance; isoforms containing the alternative exon 4b are required for anchoring mitochondrial nucleoids to the inner membrane.
  Related references: PMID:18158317, PMID:20974897
• OPA1 is involved in cristae remodeling and facilitates cytochrome c release during apoptosis, linking mitochondrial dynamics to programmed cell death pathways.
• Highly expressed in retina, with additional expression in brain, testis, heart, and skeletal muscle; mutations in OPA1 are associated with optic atrophy, deafness, and neurodegenerative disorders.
• OPA1 localizes to the mitochondrial inner membrane and intermembrane space.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein corresponding to amino acids 210-573 of human OPA1 (NP_056375.2). When performing Western blot, anticipate a band around 108–118 kDa; consider using a 6–8% SDS-PAGE gel for optimal resolution.
• For immunoprecipitation (IP) applications, validate the antibody with your specific cell or tissue lysate and optimize cross-linking conditions if necessary.
• For immunofluorescence/immunocytochemistry (IF/ICC), ensure proper fixation (e.g., paraformaldehyde) and permeabilization (e.g., Triton X-100) to allow antibody access to mitochondrial epitopes. Validate mitochondrial counterstaining in your system.
• This antibody may be suitable for ELISA against recombinant OPA1 or peptide fragments; however, appropriate coating and detection conditions should be established in-house.
• Given OPA1’s critical role in mitochondrial fusion and cristae maintenance, it may serve as a useful tool in studies of mitophagy, apoptosis, mitochondrial dynamics, and neurodegenerative disease models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. With a focus on OPA1 and mitochondrial biology, we provide one-stop access to rare-target raw materials, helping streamline your assay development from concept to clinical application. Partner with CamelBio for reliable, scalable sourcing solutions that accelerate your diagnostic and research workflows.