Anti-ONECUT1 Polyclonal Antibody for WB - Q9UBC0

Core Product Specifications and Parameters

Biological Background: ONECUT1 Function and Localization

• ONECUT1 (also known as HNF6, HNF-6, HNF6A) is a hepatocyte nuclear factor and member of the one cut domain family, acting as a transcriptional activator.
• It binds to the consensus DNA sequence 5'-DHWATTGAYTWWD-3' in the promoter regions of target genes, including HNF3B and TTR, to regulate gene expression.
• Plays a critical role in liver gene transcription and is important for liver development and function.
• Localizes predominantly to the nucleus, consistent with its function as a DNA-binding transcription factor.
• Highly expressed in the liver, with lower expression levels detected in testis and skin.
• Contains a homeobox domain and one cut domain, characteristic of DNA-binding and transcription regulation activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 167-292 of human ONECUT1, a region predicted to be accessible in the native protein; this design may support detection in applications such as Western blotting.
• For Western blot, a band of approximately 51 kDa is expected based on the calculated molecular weight; verify the observed molecular weight in your specific experimental system, as post-translational modifications may cause slight shifts.
• This antibody reacts with human, mouse, and rat species, making it potentially suitable for cross-species studies; always validate cross-reactivity in the relevant sample matrix.
• For ELISA applications, titrate the antibody and use appropriate blocking buffers to minimize nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, including this ONECUT1 polyclonal antibody, to support diagnostic manufacturers and researchers. We offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to help streamline assay development. Contact us for your liver disease and transcription factor research needs.