Anti-Oncostatin M Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P13725

Core Product Specifications and Parameters

Biological Background: Oncostatin M Function and Localization

• Oncostatin M is a multifunctional cytokine that signals via two heterodimeric receptor complexes: the type I OSM receptor (LIFR/IL6ST) and the type II OSM receptor (OSMR/IL6ST) [PubMed:10997905, PubMed:39532904, PubMed:8999038]. Related references: PMID:10997905, PMID:39532904, PMID:8999038
• It regulates a broad range of biological processes, including cell proliferation, differentiation, and cytokine production (PubMed:10997905, PubMed:1542792, PubMed:1542793). Related references: PMID:10997905, PMID:1542792, PMID:1542793
• Ligand binding induces heterodimerization of the receptor chains, activating associated Janus kinases (JAK1, JAK2, and to a lesser extent TYK2) that phosphorylate the intracellular domains of the receptors (PubMed:9188471, PubMed:8999038). Related references: PMID:9188471, PMID:8999038
• Tyrosine-phosphorylated receptors serve as docking sites for STAT3; the type II receptor additionally recruits STAT5B (PubMed:9188471). Related references: PMID:9188471
• Type II OSM receptor engagement also leads to JAK1-dependent recruitment of SHC1 via OSMR, promoting SHC1-GRB2 association and activation of the Ras/Raf/MAPK pathway (PubMed:11016927). Related references: PMID:11016927
• Oncostatin M is a secreted glycoprotein containing disulfide bonds and is synthesized as a precursor that undergoes proteolytic cleavage.
• It belongs to the interleukin-6 family of cytokines and possesses growth-regulatory and mitogenic activities.

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal peptide (aa 1-100). Consider that it may detect both full-length and proteolytically processed forms of Oncostatin M.
• In Western blot, the predicted molecular weight of the unprocessed protein is approximately 28 kDa; validate the observed band in your sample system.
• For immunofluorescence detection of this secreted cytokine, incorporate a protein transport inhibitor (e.g., brefeldin A) during cell culture to retain intracellular protein, or optimize staining for extracellular/secreted forms.
• Cross-reactivity is reported for human, mouse, and rat; however, sequence homology in the immunogen region should be verified for each species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined pathway to high-quality IVD raw materials, technical services, and consulting—from early-stage development to clinical application. This Oncostatin M monoclonal antibody is part of our cytokine and inflammation panel, and we can also supply validated antibody pairs, bulk ancillary reagents, and custom rare-target sourcing to accelerate your assay development and scale-up. Partner with us to secure consistent, reliable materials for your diagnostic pipeline.