Anti-OMA1 Rabbit Polyclonal Antibody for WB and ELISA - Q96E52

Core Product Specifications and Parameters

Biological Background: OMA1 Function and Localization

• OMA1 is a mitochondrial inner membrane zinc metalloprotease that is activated by various mitochondrial stresses to cleave OPA1, UQCC3, and DELE1. Related references: PMID:20038677, PMID:25605331, PMID:32132706
• Cleavage of OPA1 at the S1 site generates soluble OPA1 (S-OPA1), which cooperates with the long form to mediate mitochondrial inner membrane fusion. Under loss of membrane potential, OMA1 cleaves OPA1 excessively, producing S-OPA1 that inhibits fusion. Related references: PMID:31922487, PMID:20038677, PMID:25275009
• In apoptosis, OMA1 mediates OPA1 cleavage upon BAK/BAX aggregation, leading to cristae remodeling and cytochrome c release. Related references: PMID:25275009
• OMA1 activates the integrated stress response by cleaving DELE1 to produce S-DELE1, which translocates to the cytosol and activates the kinase EIF2AK1/HRI. Related references: PMID:32132706, PMID:32132707
• OMA1 can also cleave PINK1 as a backup protease, promoting its proteasomal degradation in depolarized mitochondria, and cleaves UQCC3 in response to mitochondrial depolarization. Related references: PMID:30733118, PMID:25605331
• The protein is widely expressed, with highest levels in heart, skeletal muscle, kidney, and liver. It contains a zinc-binding motif, undergoes autocatalytic processing from a zymogen, binds cardiolipin, and is required for the stability of respiratory supercomplexes.

Experimental Guidance and Technical Tips

• For Western blotting, consider using mitochondria-enriched lysates and validate detection of the ~60 kDa band corresponding to full-length OMA1; processing may produce lower molecular weight fragments under stress conditions.
• The antibody is raised against a synthetic peptide (aa 421–520) near the C-terminus; it cross-reacts with mouse and rat OMA1, enabling rodent model studies—validate specificity in your model system.
• In ELISA applications, pre-incubation with the immunogenic peptide can be used to confirm signal specificity.
• Since OMA1 activation is stress-dependent, treatment with mitochondrial depolarizing agents (e.g., CCCP) may enhance detection in functional assays; appropriate controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-OMA1 antibody for mitochondrial research. From rare-target sourcing to bulk ancillary reagents, our one-stop supply chain supports the entire development workflow—from concept to clinical application.