Anti-OLR1 Rabbit Polyclonal Antibody for WB, ELISA - P78380

Core Product Specifications and Parameters

Biological Background: Oxidized Low-Density Lipoprotein Receptor 1 (OLR1) Function and Localization

• Official full name: Oxidized low-density lipoprotein receptor 1, also known as LOX-1, C-type lectin domain family 8 member A, and lectin-like oxidized LDL receptor 1; encoded by the OLR1 gene.
• Serves as the principal receptor for recognizing, internalizing, and degrading oxidatively modified LDL (oxLDL) on vascular endothelial cells, a key event in atherosclerotic plaque initiation.
• Binding of oxLDL activates NF-kappa-B via intracellular reactive oxygen species, promoting pro-inflammatory responses, reduced nitric oxide bioavailability, monocyte adhesion, and endothelial apoptosis.
• Functions additionally as a receptor for HSP70, enabling antigen cross-presentation in dendritic cells, and acts as a leukocyte adhesion molecule during endotoxin-induced inflammation.
• Recognizes diverse ligands including advanced glycation end products, activated platelets, monocytes, apoptotic cells, and both Gram-positive and Gram-negative bacteria; may also serve as a receptor for Neisseria meningitidis adhesin A.
• Localizes primarily to the cell membrane and membrane rafts; a secreted form is also detected. Highly expressed in endothelial cells and vascular-rich tissues (placenta, lung, liver, brain, aortic intima) as well as dendritic cells.
• Undergoes alternative splicing; contains a coiled-coil domain, disulfide bonds, and is modified by palmitoylation and N-linked glycosylation, consistent with its classification as a transmembrane glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human OLR1; consider using peptide competition assays to confirm antibody specificity in your system.
• For Western blot, protein lysates from placenta, lung, or liver—tissues with high OLR1 expression—may serve as positive controls; molecular weight is approximately 31 kDa.
• ELISA protocols may benefit from optimizing coating antigen and antibody dilutions using recombinant OLR1 protein or peptide standards.
• Cross-reactivity with mouse OLR1 is reported; validate performance if planning to analyze mouse samples alongside human ones.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs by providing a comprehensive suite of IVD raw materials, including this OLR1 polyclonal antibody for atherosclerosis and cardiovascular biomarker studies. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to streamline your assay development from concept to clinic. Partner with us to secure reliable, high-quality materials tailored to your next-generation diagnostic workflows.