Anti-Olig2 Polyclonal Antibody for WB, IHC-P, ELISA - Q13516

Core Product Specifications and Parameters

Biological Background: Olig2 Function and Localization

• Olig2 (Oligodendrocyte transcription factor 2) is a class B/E basic helix-loop-helix (bHLH) transcription factor critical for specifying oligodendrocyte and motor neuron fates in the developing spinal cord and hindbrain.
• Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube and acts as an antagonist of V2 interneuron and NKX2-2-induced V3 interneuron development.
• Orchestrates progenitor cell fates during cortical gliogenesis and gliomagenesis; in cooperation with ZNF488, promotes oligodendrocyte differentiation.
• Activates oligodendrocyte precursor cell (OPC) formation while repressing olfactory bulb interneuron intermediate progenitor generation through transcriptional silencing of GSX2 enhancer elements.
• Localizes primarily to the nucleus but can also be detected in the cytoplasm.
• Expressed in the brain, with strong expression in oligodendrogliomas; expression in astrocytomas is weak to moderate, and highly variable in glioblastomas.
• Key post-translational modifications include ubiquitin conjugation; classified as a DNA-binding developmental protein and proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (aa 1-80) of human Olig2; predict reactivity with full-length protein in WB and IHC-P.
• For Western blotting, expect a band at approximately 32 kDa; optimize loading and blocking conditions for your sample type.
• When using for immunohistochemistry (paraffin-embedded tissues), consider antigen retrieval methods and validate staining patterns in positive control tissues (e.g., brain sections).
• This polyclonal antibody cross-reacts with mouse and rat Olig2; verify specificity in your target species and model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-Olig2 pAb, and bulk ancillary reagents. Our comprehensive sourcing services support neurodevelopmental and oncology research by connecting you with rare-target raw materials and tailored procurement solutions. Let CamelBio accelerate your development from concept to clinic.