Anti-Olig2 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q13516

Core Product Specifications and Parameters

Biological Background: Olig2 Function and Localization

• Encodes a basic helix-loop-helix (bHLH) transcription factor (Class B bHLHb1 / Class E bHLHe19) essential for oligodendrocyte and motor neuron specification in the spinal cord, as well as hindbrain somatic motor neuron development.
• Cooperates with OLIG1 to establish the pMN progenitor domain of the embryonic neural tube and, together with ZNF488, drives oligodendrocyte differentiation.
• Orchestrates cortical gliogenesis and gliomagenesis by activating oligodendrocyte precursor cell (OPC) formation while repressing olfactory bulb interneuron intermediate progenitor generation through transcriptional silencing of GSX2 enhancers.
• Acts as an antagonist of V2 interneuron and NKX2-2‑induced V3 interneuron development.
• Localizes predominantly to the nucleus, with additional cytoplasmic distribution.
• Highly expressed in oligodendrogliomas; expression is weak to moderate in astrocytomas and highly variable in glioblastomas.
• Classified as a developmental protein, DNA‑binding transcription factor, and proto‑oncogene with reported ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 168–323, a C‑terminal region of human OLIG2; confirm epitope accessibility in your assay when using endogenous or full‑length OLIG2.
• For Western blot, a band near 32 kDa is expected; optimize loading controls and transfer conditions for reliable detection.
• For immunofluorescence and immunohistochemistry, permeabilization may be necessary due to nuclear localization; validate antibody dilution and blocking in your specific tissue or cell system.
• In ELISA, consider direct or capture formats; the antibody’s performance may depend on coating antigen conformation and detection system.
• Cross‑reactivity with mouse and rat OLIG2 makes this antibody suitable for rodent model studies; verify cross‑reactivity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to premium IVD raw materials. This anti‑OLIG2 monoclonal antibody supports neuroscience and oncology research, particularly in oligodendrocyte biology and glioma studies. Leverage our comprehensive supply of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing to accelerate your assay development from concept to clinic.