Anti-OLFM4 Rabbit Polyclonal Antibody for WB and ELISA - Q6UX06

Core Product Specifications and Parameters

Biological Background: Olfactomedin-4 Function and Localization

• Olfactomedin-4 (OLFM4), also known as GW112, hGC-1, and hOLfD, is a secreted glycoprotein belonging to the olfactomedin family, characterized by a coiled-coil domain and multiple disulfide bonds.
• OLFM4 promotes proliferation of pancreatic cancer cells by facilitating the transition from S to G2/M phase of the cell cycle, suggesting a role in tumor progression.
• In myeloid leukemic cell lines, OLFM4 inhibits cell growth and induces both cell differentiation and apoptosis, indicating context-dependent functions in hematological malignancies.
• OLFM4 may inhibit phosphorylation and deactivation of the translation initiation factor EIF4EBP1, implicating it in the regulation of protein synthesis and cell growth.
• The protein facilitates cell adhesion, likely through interactions with cell surface lectins and cadherins, contributing to cellular attachment and tissue organization.
• OLFM4 localizes to the extracellular space and is secreted, but has also been detected in mitochondria, indicating potential dual localization and function.
• Tissue expression: OLFM4 is highly expressed in bone marrow neutrophils (higher than peripheral blood), prostate, small intestine, and colon, with moderate expression in stomach; overexpression is observed in some pancreatic cancer tissues.
• The mature protein has a molecular weight of approximately 57–58 kDa (UniProt: 57280 Da) and undergoes glycosylation, as indicated by its glycoprotein annotation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central region of OLFM4 (aa 211-510), which may recognize both full-length OLFM4 and potential isoforms or post-translationally modified forms. Consider validating specificity using OLFM4-overexpressing or knockdown cell lysates.
• For Western blotting, the predicted molecular weight is ~57 kDa; ensure adequate separation on SDS-PAGE and use appropriate blocking and antibody dilution conditions (to be optimized empirically).
• The antibody has been tested in Western blot (WB) and ELISA; for other applications such as immunohistochemistry or immunofluorescence, further validation is required.
• Cross-reactivity with mouse and rat OLFM4 is reported, making it suitable for studies in rodent models; however, sequence homology in the immunogen region should be verified for each species.
• Since OLFM4 is a secreted protein, consider analyzing cell culture supernatants or biological fluids in addition to cell lysates, and include appropriate positive controls like prostate or colon tissue extracts.

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