Anti-OLFM1 Rabbit Polyclonal Antibody for WB and ELISA - Q99784

Core Product Specifications and Parameters

Biological Background: OLFM1 Function and Localization

• Also known as Noelin, Neuronal olfactomedin-related ER localized protein, or Olfactomedin-1; belongs to the olfactomedin family.
• Functions in the regulation of axonal growth in the CNS by inhibiting the interaction between RTN4R and LINGO1, thereby preventing RTN4R-mediated axon growth cone collapse (By similarity).
• Plays a role in the production of neural crest cells from the neural tube (By similarity).
• May be required for normal olfactory responses (By similarity).
• Subcellular localization includes secretion, synapse, endoplasmic reticulum, cell projection, axon, and perikaryon.
• Post-translational modifications: N-linked glycoprotein with disulfide bonds; exists in alternative splicing isoforms.
• Key biological processes: developmental protein involved in neurogenesis and synaptic organization.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing amino acids 90-220 of human OLFM1 (NP_055094.1). This central region may detect multiple isoforms if alternative splicing occurs outside this segment.
• For Western blotting, a band around 55 kDa is expected; validate specificity using positive (e.g., brain tissue lysate) and negative controls.
• The antibody is validated for human and mouse reactivity; cross-reactivity with other species should be tested empirically.
• For ELISA, consider pairing with a monoclonal capture antibody recognizing a distinct epitope to develop a sandwich assay system.
• Optimal working dilutions must be determined experimentally for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for procuring high-quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This OLFM1 polyclonal antibody supports research in neuroscience and developmental biology, focusing on axonal guidance and neural crest development. Leverage CamelBio’s supply chain expertise to streamline your diagnostic development with reliable raw materials and technical support.