Anti-OGT Monoclonal Antibody for WB, ELISA, ChIP - O15294

Core Product Specifications and Parameters

Biological Background: OGT Function and Localization

• OGT (UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit) is the catalytic subunit of the O-GlcNAc transferase complex, responsible for adding a single N-acetylglucosamine (O-GlcNAc) to serine/threonine residues of nuclear and cytoplasmic proteins (PubMed:12150998, PubMed:15361863, PubMed:19451179). Related references: PMID:12150998, PMID:15361863, PMID:19451179
• It glycosylates a vast array of substrates, including histone H2B, AKT1, AMPK, EZH2, MAPT/TAU, and many others, thereby regulating key processes such as transcription, insulin signaling, autophagy, and circadian rhythms (PubMed:19451179, PubMed:20200153, PubMed:21285374). Related references: PMID:19451179, PMID:20200153, PMID:21285374
• OGT exists in multiple isoforms, including a mitochondrial isoform (mOGT) that is cytotoxic and promotes apoptosis, particularly in insulinoma cells. It also has a specialized N-acetylglucosaminyltransferase activity with distinct substrate preference (PubMed:31527085). Related references: PMID:31527085
• The enzyme localizes to the nucleus, cytoplasm, mitochondria, and cell membrane, reflecting its diverse functional roles.
• Post-translational modifications of OGT include acetylation and phosphorylation, and it participates in ubiquitin conjugation pathways. Keywords also highlight its involvement in apoptosis, biological rhythms, chromatin regulation, and host-virus interactions.
• OGT is highly expressed in the pancreas, with moderate levels in skeletal muscle, heart, brain, and placenta, and trace amounts in lung and liver, aligning with its role in insulin signaling and glucose homeostasis.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to residues 947–1046 of human OGT, a region likely exposed in the native protein; consider using this antibody for denaturing Western blot where the epitope is accessible.
• For ChIP assays, validate cross-linking conditions and antibody specificity with appropriate positive and negative controls, given OGT’s chromatin association.
• In Western blot, the predicted molecular weight is ~117 kDa; however, multiple isoforms and extensive post-translational modifications may result in additional bands or shifts; include proper molecular weight markers.
• When working with rodent samples, confirm reactivity in mouse or rat tissues, as the antibody shows cross-reactivity; optimization of dilution and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw materials, offering this high-quality anti-OGT monoclonal antibody alongside a broad portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities, optimized clones for O-GlcNAcylation or chromatin research, or assistance in sourcing rare targets, we bridge the gap from concept to clinic. Contact us to discuss your project-specific requirements and accelerate your diagnostic development.