Anti-OGG1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O15527

Core Product Specifications and Parameters

Biological Background: OGG1 Function and Localization

• OGG1 (also known as HMMH, MUTM, OGH1) encodes an N-glycosylase/DNA lyase that functions in the base excision repair pathway, specifically excising 7,8-dihydro-8-oxoguanine (8-oxoG) and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA.
• The enzyme possesses an associated beta-lyase activity that nicks DNA at the 3' side of the lesion, facilitating removal of the damaged base.
• Subcellularly, OGG1 localizes to both the nucleus and mitochondria; within the nucleus it is found in the nucleoplasm, nuclear speckles, and nuclear matrix, consistent with its DNA repair roles.
• Expression is ubiquitous across tissues, underscoring its importance in maintaining genomic integrity.
• Multiple isoforms are generated by alternative splicing (as indicated by the keyword 'Alternative splicing'), potentially allowing functional specialization.
• UniProtKB keywords highlight its involvement in DNA damage and repair pathways, and its classification as a multifunctional enzyme (glycosidase, hydrolase, lyase).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-345 of human OGG1 (NP_002533.1), covering the full-length protein; thus the antibody may recognize multiple isoforms.
• For Western blot (WB), the predicted molecular weight is approximately 39 kDa; however, alternative splicing products or post-translational modifications may result in additional bands.
• IHC-P detection has been validated, but optimal conditions (e.g., antigen retrieval, antibody dilution) should be determined experimentally using appropriate positive control tissues (e.g., human tonsil, colon).
• This antibody cross-reacts with mouse and rat OGG1, allowing its use in rodent models; however, sequence homology should be checked if working with other species.
• For ELISA, consider using recombinant human OGG1 protein as standard and validate the assay linearity and sensitivity in your specific matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. Our OGG1 rabbit polyclonal antibody supports DNA damage/repair research and assay development. Beyond this product, we offer one-stop sourcing for rare-target raw materials and bulk supply, helping you accelerate from concept to clinic with reliable, high-quality components.