Anti-OGFR Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NZT2

Core Product Specifications and Parameters

Biological Background: OGFR Function and Localization

• OGFR (opioid growth factor receptor), also designated protein 7-60 and zeta-type opioid receptor, serves as the receptor for opioid growth factor (OGF/Met-enkephalin).
• The receptor is implicated in the regulation of cellular proliferation and tissue growth, acting through endogenous opioid pathways.
• OGFR localizes to both the cytoplasm and the nucleus, suggesting roles in cytoplasmic signaling and nuclear gene regulation.
• It is highly expressed in heart and liver, with moderate levels in skeletal muscle and kidney, and lower expression in brain and pancreas; fetal tissues also express the protein.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics, which may influence receptor function and stability.
• Alternative splicing generates multiple transcript variants, though their specific biological significance is not fully defined.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 358–482 of human OGFR, a C-terminal region that may contain distinct epitopes; consider its potential cross-reactivity with isoforms.
• For Western blot, the predicted molecular weight of OGFR is approximately 73 kDa; validate band specificity using appropriate positive and negative controls.
• In immunohistochemistry and immunofluorescence experiments, expect both cytoplasmic and nuclear staining patterns; optimize antigen retrieval and antibody dilution for each tissue type.
• ELISA may be utilized for quantitative detection; verify performance using recombinant protein standards and relevant biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including validated antibodies, antigen pairs, and bulk reagents. This anti-OGFR polyclonal antibody supports research into opioid growth factor signaling and growth regulation. Partner with us to streamline your diagnostic development with one-stop procurement and technical consultation services.