Anti-OGDH Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q02218

Core Product Specifications and Parameters

Biological Background: OGDH Function and Localization

• OGDH encodes the E1o subunit of the 2-oxoglutarate dehydrogenase complex (OGDHC), catalyzing the rate-limiting, irreversible decarboxylation of 2-oxoglutarate to succinyl-CoA in the citric acid cycle. Related references: PMID:24495017 PMID:25210035 PMID:28435050

• The reaction requires the cofactor thiamine diphosphate (ThDP) and transfers the decarboxylated intermediate to the E2 enzyme (DLST) for further conversion. Related references: PMID:24495017 PMID:25210035 PMID:28435050

• OGDH can also decarboxylate 2-oxoadipate in vitro, albeit at a lower efficiency, and may process other related substrates. Related references: PMID:28435050

• Beyond its mitochondrial role, a nuclear pool of OGDHC supplies succinyl-CoA to the histone succinyltransferase KAT2A, regulating chromatin succinylation. Related references: PMID:29211711

• Alternative splicing yields multiple isoforms of OGDH, contributing to functional diversity.

• The protein is subject to acetylation, phosphorylation, and ubiquitin-like modifications, as identified by proteomics.

• OGDH activity depends on calcium and magnesium ions, linking energy metabolism to cellular signaling.

• As a central Krebs cycle enzyme, OGDH integrates the oxidation of carbohydrates, fatty acids, and amino acids. Related references: PMID:25210035

Experimental Guidance and Technical Tips

• The polyclonal antibody, generated against a recombinant fragment (aa 148-427), may detect multiple OGDH isoforms and is likely suitable for detecting endogenous OGDH across human, mouse, and rat samples.
• For Western blot, a major band near 116 kDa is expected; minor bands corresponding to alternative isoforms or cleavage products may also appear.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), both mitochondrial and nuclear localization patterns should be considered, with appropriate controls.
• This antibody can be used in ELISA for quantitative analysis; users should optimize coating and detection conditions.
• Cross-reactivity with mouse and rat homologs has been indicated, but validation in the relevant experimental model is recommended.

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