Anti-OGDH Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q02218

Core Product Specifications and Parameters

Biological Background: OGDH Function and Localization

• OGDH encodes the E1o component (2-oxoglutarate dehydrogenase) of the multienzyme 2-oxoglutarate dehydrogenase complex (OGDHC), also known as alpha-ketoglutarate dehydrogenase (Alpha-KGDH-E1). Related references: PMID:24495017 PMID:25210035 PMID:28435050
• Catalyzes the rate-limiting, irreversible decarboxylation of 2-oxoglutarate using thiamine diphosphate (ThDP) and transfers the succinyl intermediate to the E2 subunit (DLST), a key step of the Krebs cycle. Related references: PMID:24495017 PMID:25210035 PMID:28435050
• Primarily localizes to the mitochondrial matrix, but a fraction of the OGDHC resides in the nucleus where it supplies succinyl-CoA for histone succinylation by KAT2A. Related references: PMID:29211711
• Can also decarboxylate 2-oxoadipate in vitro, though with lower efficiency, and may act on 2-keto-4-hydroxyglutarate (KHG) to form malyl-CoA. Related references: PMID:28435050
• The protein is subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, and contains calcium- and magnesium-binding sites.
• Alternative splicing generates multiple transcript variants, and a transit peptide directs the precursor to mitochondria before cleavage to mature form.

Experimental Guidance and Technical Tips

• The immunogen (aa 148–427) covers a central region of OGDH, enabling recognition of both full-length and potential cleavage products; confirm detection of the expected ~116 kDa band in relevant lysates.
• For Western blot (WB), consider loading appropriate mitochondrial-enriched or whole-cell extracts and use a 6–8% SDS-PAGE gel for optimal resolution of high-molecular-weight proteins.
• When performing IHC-P or IF/ICC, include a permeabilization step to allow antibody access to mitochondrial and nuclear compartments; validate signal specificity with appropriate controls.
• In ELISA, use the antibody as a capture or detection reagent after confirming linear range and sensitivity with recombinant OGDH protein or lysate standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers, labs, and research institutes, offering validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For OGDH-related projects, we supply anti-OGDH rabbit pAb for mitochondrial metabolism and Krebs cycle studies, along with complementary antibodies, antigens, and technical support to accelerate your development workflow.