Anti-OGDH Monoclonal Antibody for WB, IHC-P, ELISA - Q02218

Core Product Specifications and Parameters

Biological Background: OGDH Function and Localization

• OGDH encodes the E1o component of the 2-oxoglutarate dehydrogenase complex (OGDHC), catalyzing the rate-limiting, irreversible decarboxylation of 2-oxoglutarate to succinyl-CoA and CO₂ via a thiamine diphosphate (ThDP) cofactor. Related references: PMID:24495017 PMID:25210035 PMID:28435050
• It plays a central role in the Krebs (citric acid) cycle, a common pathway for oxidation of carbohydrates, fatty acids, and amino acids. Related references: PMID:25210035
• In vitro, OGDH can decarboxylate 2-oxoadipate, though at a much lower rate than 2-oxoglutarate, and can convert 2-keto-4-hydroxyglutarate (KHG) and CoA into malyl-CoA.
• A fraction of OGDH localizes to the nucleus, where it associates with KAT2A on chromatin and supplies succinyl-CoA for histone succinylation, linking mitochondrial metabolism to epigenetic regulation. Related references: PMID:29211711
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, as identified in proteomics studies.
• Alternative splicing generates multiple isoforms, potentially modulating enzymatic activity or subcellular distribution.
• The enzyme requires thiamine pyrophosphate, calcium, and magnesium as cofactors for full catalytic function.

Experimental Guidance and Technical Tips

• The immunogen covers the C‑terminal region (aa 869‑1023). Consider verifying antibody specificity for endogenous OGDH in your cell or tissue models, as post‑translational modifications or binding partners may obscure the epitope.
• For western blotting, OGDH migrates at approximately 116 kDa; validate detection with appropriate controls and note that mitochondrial processing can affect apparent molecular weight.
• In immunohistochemistry (paraffin sections), use of heat‑induced epitope retrieval is recommended; nuclear as well as mitochondrial staining may be observed.
• For ELISA applications, consider pairing this monoclonal antibody with a detection antibody that recognizes a distinct epitope to avoid competition.
• Cross‑reactivity with mouse and rat is indicated; if using these species, confirm reactivity in the relevant sample system and consider that sequence homology may affect sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials. This anti‑OGDH antibody supports research into the Krebs cycle, metabolic disorders, and epigenetic mechanisms. Beyond individual monoclonal antibodies, we offer validated antibody pairs, optimized recombinant immunoreagents, and bulk ancillary reagents. For rare or custom targets, our sourcing team can connect you with reliable supplies to accelerate your development from concept to clinic.