Anti-ODF1 Polyclonal Antibody for WB and ELISA - Q14990

Core Product Specifications and Parameters

Biological Background: ODF1 Function and Localization

• ODF1 (Outer dense fiber protein 1) is also known as Heat shock protein beta-10 (HspB10) and is a member of the heat shock protein family B member 10.
• It is a major component of the outer dense fibers (ODF) of spermatozoa, which are filamentous structures located outside the axoneme in the midpiece and principal piece of the sperm tail.
• ODFs contribute to the passive elastic structures and elastic recoil of the sperm tail, essential for sperm motility.
• The protein is specifically expressed in the testis, consistent with its role in spermatogenesis and sperm differentiation.
• Subcellularly, ODF1 localizes to the sperm flagellum, including the axoneme and centrosome, as well as to the cytoplasm and cytoskeleton.
• Keywords associated with ODF1 include cell projection, cilium, flagellum, cytoskeleton, developmental protein, differentiation, phosphoprotein, spermatogenesis, and repeat regions, indicating its structural and regulatory roles in sperm development.

Experimental Guidance and Technical Tips

• The immunogen comprises the central region (aa 80-180) of human ODF1, which is expected to generate antibodies recognizing both the native and denatured protein.
• For Western blot (WB), consider using reduced and non-reduced sperm or testis lysates. A band near 28 kDa is expected; note that ODF1 may form higher-order structures under non-reducing conditions.
• For ELISA, the antibody can be used for capture or detection of recombinant or endogenous ODF1; however, cross-reactivity with mouse and rat orthologs may allow multi-species applications with appropriate validation.
• Given the testis-specific expression, it is advisable to include negative controls from non-testicular tissues to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio supports diagnostic manufacturers with a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies such as this anti-ODF1 antibody.
• For reproductive biology and fertility diagnostics, we offer antibody pairs, ancillary reagents, and sourcing assistance for rare targets like ODF1 to streamline your assay development.
• As a one-stop sourcing bridge, CamelBio provides reliable access to high-quality raw materials, technical services, and consulting from concept to clinical application.