Anti-ODC1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P11926

Core Product Specifications and Parameters

Biological Background: ODC1 Function and Localization

• Ornithine decarboxylase (ODC1) catalyzes the first and rate-limiting step of polyamine biosynthesis, converting ornithine into putrescine, the precursor for spermidine and spermine.
• Polyamines are essential for cell proliferation and are implicated in diverse cellular processes, from DNA replication to apoptosis.
• ODC1 functions as a pyridoxal phosphate-dependent decarboxylase, belonging to the lyase enzyme family.
• The protein undergoes post-translational modifications, including phosphorylation and S-nitrosylation, which may regulate its activity.
• Disease-associated mutations in ODC1 have been linked to hypotrichosis, highlighting its importance in normal tissue development.
• According to UniProt, ODC1 is part of the reference proteome, and its three-dimensional structure has been resolved.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–280) of human ODC1; ensure your target epitope is preserved under your experimental conditions.
• For Western blot, the predicted molecular weight is approximately 51 kDa; consider using appropriate gel percentages and transfer conditions.
• The antibody is suitable for WB, IF/ICC, and ELISA; cross-reactivity with mouse and rat ODC1 has been noted, but validation in your specific sample system is recommended.
• When performing immunofluorescence, optimize permeabilization and blocking steps for detection of this intracellular enzyme.
• For ELISA, consider pairing with a suitable capture or detection reagent for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-ODC1 antibody, and bulk ancillary reagents to support polyamine metabolism and cell proliferation research. With end-to-end technical services and consulting, CamelBio streamlines your journey from concept to clinic.