Anti-OCT4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q01860

Core Product Specifications and Parameters

Biological Background: OCT4 (POU5F1) Function and Localization

• OCT4, also known as Oct-3, Oct-4, or OTF-3, is a POU domain transcription factor derived from the POU5F1 gene. It is a key developmental protein with multiple isoforms.
• The protein binds specifically to the octamer motif (5'-ATTTGCAT-3') on DNA, regulating gene expression.
• OCT4 forms a trimeric complex with SOX2 or SOX15 on DNA, controlling the expression of critical embryonic development genes such as YES1, FGF4, UTF1, and ZFP206.
• It is indispensable for early embryogenesis and for the maintenance of embryonic stem cell pluripotency.
• Subcellularly, OCT4 localizes to both the cytoplasm and the nucleus, consistent with its role as a transcription factor.
• Tissue expression is predominantly observed in the developing brain, with high levels in specific cell layers of the cortex, olfactory bulb, hippocampus, and cerebellum. Expression is low in adult tissues.
• The protein is subject to post-translational modifications, including phosphorylation and ubiquitination (Ubl conjugation), which may regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from amino acids 210-310 of human OCT4. As a polyclonal antibody, it may recognize multiple isoforms or related family members; validate specificity in your experimental system.
• For Western blot (WB), the predicted molecular weight is approximately 39 kDa. Optimize loading controls and antibody dilutions for your specific sample type.
• In immunohistochemistry (IHC-P), consider using human or mouse embryonic stem cells or developing brain tissues as positive controls. Antigen retrieval may be required.
• For ELISA, this antibody can be used for detection; consider pairing it with a capture antibody for sandwich ELISA if needed.
• Cross-reactivity is reported with human and mouse; verify if other species are also detected if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For stem cell and developmental biology research, we supply this high-quality anti-OCT4 polyclonal antibody alongside validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare and emerging targets. Our integrated supply capabilities help streamline your procurement and accelerate project timelines.