Anti-OCRL Rabbit Polyclonal Antibody for WB, ELISA - Q01968

Core Product Specifications and Parameters

Biological Background: OCRL Function and Localization

• OCRL is an inositol polyphosphate 5-phosphatase that primarily hydrolyzes the 5-phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), with the highest catalytic activity towards PtdIns(4,5)P2. Related references: PMID:10764818, PMID:15474001, PMID:7761412

• It also hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4). Related references: PMID:15474001, PMID:40969890, PMID:7761412

• Regulates endosomal trafficking by controlling the local pool of PtdIns(4,5)P2 on endosomes. Related references: PMID:21971085

• Plays a critical role in primary cilia assembly. Related references: PMID:22228094, PMID:22543976

• Involved in phagocytosis by hydrolyzing PtdIns(4,5)P2 to promote phagosome closure through attenuation of PI3K signaling. Related references: PMID:22072788

• Localizes to multiple cellular compartments, including the trans-Golgi network, endosomes, lysosomes, clathrin-coated pits, and the primary cilium.

• Highly expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, and retinal tissues.

• Mutations in OCRL cause Lowe syndrome (oculocerebrorenal syndrome) and Dent disease, characterized by cataracts, renal dysfunction, and neurological abnormalities.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 104 kDa corresponding to full-length OCRL. Use a positive control lysate from brain, kidney, or retinal tissues.
• The immunogen covers amino acids 1–220 of human OCRL; thus, the antibody may recognize both full-length and N-terminal-containing isoforms. Verify detection of known isoforms in your sample system.
• When working with mouse or rat samples, confirm sequence homology of the immunogen region to ensure cross-reactivity.
• For ELISA, consider using the recombinant immunogen or purified OCRL protein as a standard.
• Optimize the antibody dilution for your specific application and sample type; perform a dose-response curve if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. The OCRL antibody targets a critical enzyme in phospholipid metabolism and ciliary biology, serving as a valuable tool for research into Lowe syndrome and related ciliopathies. Our one-stop sourcing platform streamlines procurement, from concept to clinic, with reliable supply and technical consultation.