Anti-OCRL Rabbit Polyclonal Antibody for WB and ELISA - Q01968

Core Product Specifications and Parameters

Biological Background: OCRL Function and Localization

• OCRL is an inositol polyphosphate 5-phosphatase encoded by the OCRL gene (also known as OCRL1). It catalyzes the hydrolysis of the 5-position phosphate from phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), with highest activity towards PtdIns(4,5)P2 (PubMed:10764818, PubMed:15474001, PubMed:7761412).
  Related references: PMID:10764818, PMID:15474001, PMID:7761412
• It also hydrolyzes inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate (PubMed:15474001, PubMed:40969890, PubMed:7761412).
  Related references: PMID:15474001, PMID:40969890, PMID:7761412
• OCRL regulates endosomal trafficking by modulating the pool of PtdIns(4,5)P2 associated with endosomes (PubMed:21971085).
  Related references: PMID:21971085
• It is involved in primary cilium assembly (PubMed:22228094, PubMed:22543976).
  Related references: PMID:22228094, PMID:22543976
• OCRL acts as a regulator of phagocytosis by hydrolyzing PtdIns(4,5)P2 to promote phagosome closure via attenuation of PI3K signaling (PubMed:22072788).
  Related references: PMID:22072788
• Subcellularly, OCRL localizes to the cytoplasmic vesicle, phagosome membrane, early endosome membrane, clathrin-coated pits, primary cilium, photoreceptor outer segment, endosomes, Golgi apparatus, trans-Golgi network, and lysosomes.
• It is widely expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina, and retinal pigment epithelium.
• Mutations in OCRL cause Lowe oculocerebrorenal syndrome (Dent disease 2), highlighting its role in cellular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–220) of human OCRL, suggesting this polyclonal antibody may detect full-length OCRL and N-terminal fragments. For Western blotting, consider using appropriate controls to confirm specificity.
• Given the predicted molecular weight of ~104 kDa, ensure adequate protein separation and transfer conditions for high-molecular-weight proteins.
• This antibody reacts with human, mouse, and rat OCRL; validate in the relevant species and tissue types (e.g., kidney, brain, or fibroblasts) to confirm performance.
• For ELISA applications, optimize coating antigen concentration and antibody dilution to achieve the desired sensitivity and signal-to-noise ratio.
• The antibody may be suitable for immunoprecipitation or immunofluorescence based on the target's subcellular localization; however, validation in these applications is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of high-quality antibodies and reagents tailored to diagnostics development. For researchers studying inositol phosphate metabolism, endosomal trafficking, or ciliopathies, we provide access to validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our supply network supports every stage from concept to clinic, ensuring reliable access to critical raw materials for assay development.